Journal: Scientific Reports

Article Title: Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma

doi: 10.1038/s41598-025-31872-8

Figure Lengend Snippet: ( A ). Representative images of OE-19 xenograft bearing mice imaged at 4 and 6 h post NF-800 injection at IVIS Spectrum. ( B ) Mean fluorescence intensity values of Tumor, Kidney and Liver of animals bearing OE-19 xenografts and treated with NF-800. ( C ) Lifetime values obtained with tail fitting of the abovementioned tissues, compared to the probe dissolved in PBS (n = 7 animals). One way ANOVA Multiple comparison test (* = p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001).

Article Snippet: Fluorescence intensity acquisitions were performed at IVIS Spectrum (Perkin Elmer Italia S.p.A.) equipped with an excitation filter at 745 nm and emission filter at 800 nm with 30 nm and 20 nm bandwidth, respectively.

Techniques: Injection, Fluorescence, Comparison

Journal: Scientific Reports

Article Title: Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma

doi: 10.1038/s41598-025-31872-8

Figure Lengend Snippet: ( A ) Fluorescence intensity values (counts) and respective lifetimes (ns) of ex vivo tumor and kidneys 6 h after administration of NF-800. ( B ) Fluorescence intensity values and respective lifetimes (ns) of ex vivo tumor and liver after administration of Tz-800. (One way ANOVA Multiple comparison test was performed between all the groups (e.g. tumor vs kidney; tumor vs PBS; kidney vs PBS) (* = p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001).

Article Snippet: Fluorescence intensity acquisitions were performed at IVIS Spectrum (Perkin Elmer Italia S.p.A.) equipped with an excitation filter at 745 nm and emission filter at 800 nm with 30 nm and 20 nm bandwidth, respectively.

Techniques: Fluorescence, Ex Vivo, Comparison

Journal: Scientific Reports

Article Title: Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma

doi: 10.1038/s41598-025-31872-8

Figure Lengend Snippet: Representative Fluorescence Intensity (AU) and FLT parametric map (ns) of a tumor treated with NF-800. ROIs indicate tumors, which appears to have spread throughout the stomach. Histogram represents distribution of lifetimes across pixels, as fitted to obtain the parametric map.

Article Snippet: Fluorescence intensity acquisitions were performed at IVIS Spectrum (Perkin Elmer Italia S.p.A.) equipped with an excitation filter at 745 nm and emission filter at 800 nm with 30 nm and 20 nm bandwidth, respectively.

Techniques: Fluorescence

Journal: The Analyst

Article Title: Ferguson analysis of protein electromigration during single-cell electrophoresis in an open microfluidic device

doi: 10.1039/c9an02553g

Figure Lengend Snippet: Dependence of migration distance on protein molecular mass in three different gel compositions. A) Open microfluidic device for single-cell PAGE immunoassay. Single-cells are gravity settled into an array of microwells for subsequent cell lysis and protein solubilization, protein PAGE, and in-gel immobilization of separated proteins. Protein targets are immunoprobed with fluorophore-conjugated antibodies. B) (Left) Relationship between electromigration distance and the logarithm value (base 10) of molecular mass of nine different protein targets (GAPDH, 36 kDa; β-tubulin, 50 kDa; PTBP1, 57 kDa (above limit of detection in 6/9 gels tested); ER-α, 66 kDa; HSP90, 90 kDa; SFPQ, 95 kDa; α-Actinin, 103 kDa; Vinculin, 124 kDa; and mTOR, 289 kDa from lysed MCF-7 cells. Three different gel composition conditions (5%T, 6%T and 8%T) were used to analyze the differing electrophoretic migration distances. Boxplots represent aggregation of single-cell data points taken from 3 replicate devices with the number of individual cells assayed at the bottom of each plot. Dashed lines (---) represent linear fits including mTOR, while solid lines (—) indicate fits excluding mTOR. (Right) Representative false-color fluorescence micrographs and intensity plots as examples of protein electromigration in each gel condition. (See Figure S1 for additional targets)

Article Snippet: Gaussian curves were fit to fluorescence intensity profiles in MATLAB (R2018b, Curve Fitting Toolbox) in order to obtain the mean μ (used to describe the protein migration distance) and the variance σ 2 (used to calculate peak width as 4σ).

Techniques: Migration, Lysis, Fluorescence